LOCUS       40924_44759        9420 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-R57 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Takahashi K, Ueda R.
  TITLE     Cloning vector pUAST-R57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Ueda R, Takahashi K.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2005) Kuniaki Takahashi, National Institute of 
            Genetics, Genetic Strains Research Center; 1,111 Yata, Mishima, 
            Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp, 
            Tel:81-55-981-6824, Fax:81-55-981-6825)
REFERENCE   3  (bases 1 to 9420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2005) Kuniaki Takahashi, National Institute of Genetics, 
            Genetic Strains Research Center"; volume: " 1,111 Yata, Mishima, 
            Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp, 
            Tel:81-55-981-6824, Fax"; pages: "81-55-981-6825"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3038..3132
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3151..3389
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3389..3474
                     /note="polylinker; unique cloning sites for BglII, NotI
                     CpoI, KpnI"
     CDS             join(3475..3482,3538..3676,3737..3756)
                     /codon_start=1
                     /gene="Dret"
                     /product="Ret oncogene"
                     /label=Dret
                     /note="Drosophila ret partial sequence"
                     /protein_id="BAE44178.1"
                     /translation="ELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRD
                     RVPVKWMAPE"
     gene            join(3475..3482,3538..3676,3737..3756)
                     /gene="Dret"
                     /label=Dret
     misc_feature    3757..3818
                     /note="polylinker; unique cloning sites for SfiI, XbaI"
     intron          3896..3961
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4091..4111
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4536..4670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4688..8824
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8835..9420)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
     3001 tgcatgagct cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga
     3061 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3121 ctgtcctccg agcggagact ctagcgagcg ccggagtata aatagaggcg cttcgtctac
     3181 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     3241 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     3301 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     3361 taattattga atacaagaag agaactctga atagggaatt gggaattcgt taacagatct
     3421 gcggccgcgg tacccggtcc gctcgacggt atcgataagc ttgatatcga attcgagtta
     3481 aaggtgggta agagaaataa ttttttcaac caatatatag acgcccttta tcctccagtt
     3541 ggttcatcgt gatttggctg ctagaaatgt gctccttgcg gatggcaaga tatgcaaaat
     3601 atcagatttc ggactgactc gagatgttta cgaggacgat gcctatttaa agagatcccg
     3661 agatcgtgtg cccgtcaagg taagacctca aaattaaccc cctataacat taatattaat
     3721 ccggaaaccc cttttaaagt ggatggctcc ggaatcgaat tcctgcagcc cgggggatcc
     3781 ggcctacatg gccttaattg gcctatatgg cctctagagg atctttgtga aggaacctta
     3841 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     3901 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     3961 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     4021 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     4081 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     4141 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     4201 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     4261 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     4321 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     4381 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     4441 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4501 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ggatccacta
     4681 gaaggcctta gtatgtatgt aagttaataa aacccttttt tggagaatgt agatttaaaa
     4741 aaacatattt tttttttatt ttttactgca ctggacatca ttgaacttat ctgatcagtt
     4801 ttaaatttac ttcgatccaa gggtatttga agtaccaggt tctttcgatt acctctcact
     4861 caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat
     4921 cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttaccta
     4981 gcgtcaatgt ccgccttcag ttgcactttg tcagcggttt cgtgacgaag ctccaagcgg
     5041 tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg
     5101 tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt
     5161 gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac
     5221 acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac
     5281 ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc
     5341 acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga ttattctggt
     5401 agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga catctaaagg
     5461 cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata ttttaagctg
     5521 atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcgtttt
     5581 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     5641 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     5701 tacggcacgc tcctgccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     5761 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     5821 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     5881 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     5941 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     6001 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     6061 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     6121 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     6181 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg
     6241 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     6301 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     6361 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc
     6421 cgcttctgat ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg
     6481 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     6541 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     6601 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     6661 attaagggta tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt
     6721 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     6781 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgctat tagcaaagta
     6841 gcccgggata tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg
     6901 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     6961 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     7021 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     7081 tagattttag ttagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     7141 tagtttatag ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca tgttgaagat
     7201 atgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     7261 cctcatcttt ttgggccaac aactcacgca agtgggcgtg atgaatatca acggagccat
     7321 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtct
     7381 tgtttagaat acatttgcat attaataatt tactaacttt ctaatgaatc gattcgattt
     7441 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     7501 gtgacacata ctttctgggc aaaacgattg ccgaattacc gctttttctc acagtgccac
     7561 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     7621 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     7681 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     7741 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     7801 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     7861 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     7921 gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg
     7981 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     8041 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     8101 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     8161 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     8221 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     8281 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     8341 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     8401 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     8461 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
     8521 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
     8581 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
     8641 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
     8701 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
     8761 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
     8821 ctagaattaa ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt
     8881 ttccctcaag ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat
     8941 tgtttacata aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg
     9001 tatagccttc ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg
     9061 cgttgagtcg cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat
     9121 cattaaagtg ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct
     9181 cccacaattt tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag
     9241 ctttgcagca aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt
     9301 caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt
     9361 aagtgtatac ttcggtaagc ttcggctatc gacgggacca ccttatgtta tttcatcatg
//
